Iscript cdna synthesis kit product manual

Description. The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range, with minimal setup and . The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two-step RT-PCR. The novel qScript Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. The optimized blend of random and oligo(dT) primers. the iScript cDNA Synthesis Kit The maximum amount of the cDNA reaction that is recommended for downstream PCR is one-tenth of the reaction volume, typically 2 µl. Related Products Catalog # Description Reverse Transcription Reagents for Real-Time qPCR iScript Reverse Transcription Supermix for RT-qPCR iScript Advanced cDNA Synthesis Kit for RT-qPCRMissing: product manual.

The iScript Select cDNA synthesis kit is a sensitive, flexible, and easy-to-use kit for the generation of first-strand cDNA. This robust reverse transcription kit allows a selection of first-strand priming strategies †, oligo(dT) primers only, random primers only, or user-designed gene-specific primers. This cDNA synthesis kit provides all required reagents, except RNA template and gene-specific primers, to create first-strand cDNA from an RNA template. The iScript Select cDNA synthesis kit is a sensitive, flexible, and easy-to-use kit for the generation of first-strand cDNA. This robust reverse transcription kit allows a selection of first-strand priming strategies †, oligo(dT) primers only, random primers only, or user-designed gene-specific primers. Fig. 2. The iScript Select cDNA Synthesis Kit facilitates synthesis of cDNA longer than 8 kb. First-strand cDNA was produced from 1 µg total RNA using the iScript Select cDNA Synthesis Kit and the provided oligo(dT) primer mix. A 2 µl aliquot of the 20 µl cDNA synthesis reaction was subjected to 35 PCR.

Documents Downloads · Certificates of Analysis (COA) · Material Safety Data Sheets (MSDS) · Manuals · Product Literature. Thus, refer to the PCR enzyme instruction manual to assess the appropriate amount of template to use. If non-specific amplification or no product is obtained. 20 мар. г. iScript™ Advanced cDNA Synthesis Kit (Bio-Rad) PBMCs using the QIAamp DNA Mini Kit (QIAGEN) according to manufacturer's instructions.